This site is the demo site associated with the manuscript:
And the github:
https://github.com/kallistaconsulting/genomic_resources_clogmia
We generated a high quality de novo genome assembly of Clogmia albipunctata with chromosome-size scaffolds, consistent with the known chromosome number in this species (84, 85). The total length of the chromosome-size scaffolds is 304.3 Mb, close to genome size estimates based on flow cytometry data (316.6 Mb) (86). An additional 268 small scaffolds (< 1 MB) contained mostly repetitive sequences.
We annotated this new reference genome using RNA-seq transcripts from embryonic, larval, pupal, and adult stages, protein sequences, and ab initio gene prediction software, and identified 15,047 protein coding genes. The annotated genome recovered 88%-94% of Complete Benchmarking Universal Single Copy Orthologs (BUSCO)
This genome and annotation is available on this platform, and via the above github. Additional tools are included to assist with analysis and use of this genome, including JBrowse2-integrated BLAST, CrisprDesign tools, and RNAseq analysis tools (via R shiny). Please see the tutorials on the github for usage information.